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stemmacstm ipsc mrna reprogramming kit  (Miltenyi Biotec)
94
Miltenyi Biotec stemmacstm ipsc mrna reprogramming kit
Overview of the experimental layout: focus on primary testicular somatic cell cultures and <t>reprogramming</t> . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the <t>mRNA-based</t> reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).
Stemmacstm Ipsc Mrna Reprogramming Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemmacs+mrna+reprogramming+kit/StemMACS+iPSC+mRNA+Reprogramming+Kit%2C+human/pmc13322296-78-28-33
Average 94 stars, based on 1 article reviews
stemmacstm ipsc mrna reprogramming kit - by Bioz Stars, 2026-09
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Overview of the experimental layout: focus on primary testicular somatic cell cultures and reprogramming . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the mRNA-based reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).

Journal: Human Reproduction Open

Article Title: Human iPSCs derived from cryopreserved testicular somatic cells enable germline regeneration in childhood cancer survivors

doi: 10.1093/hropen/hoag054

Figure Lengend Snippet: Overview of the experimental layout: focus on primary testicular somatic cell cultures and reprogramming . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the mRNA-based reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).

Article Snippet: Upon reaching confluency, cells were seeded at 2000 cells/cm 2 in 35 mm dishes on day-3 and transfected twice daily from day 0 to day 4 using the StemMACSTM iPSC mRNA Reprogramming Kit (Miltenyi Biotec, Bergisch Gladbach, Germany, #130-132-990).

Techniques: Isolation, Clone Assay